Dab tsi raw cov ntaub ntawv siv rau extracting sulforaphane?
Cov kev tshawb fawb tau pom tias cov nroj tsuag cruciferous Brassica, zaub qhwv, zaub qhwv, thiab kale muaj cov ntsiab lus sulforaphane siab, tshwj xeeb tshaj yog nyob rau hauv lawv cov noob. Ib txoj kev tshawb fawb sib piv tau pom tias qhov kev rho tawm ntawm sulforaphane hauv broccoli noob yog ntau dua li lwm cov noob, tab sis vim yog cov nqi siab ntawm cov noob, tus nqi rho tawm yog siab thiab nws nyuaj rau kev ua lag luam. Yog li ntawd, cov khoom siv raw siv rau kev tsim khoom txhua hnub thiab kev rho tawm yog broccoli sprouts, uas yog cov nroj tsuag hluas broccoli. Piv nrog rau cov txiv hmab txiv ntoo paub tab, broccoli sprouts muaj ntau sulforaphane, thiab ntxiv rau, cov kauj ruam extraction yog yooj yim nrog tsuas yog ob kauj ruam ntawm enzymatic digestion thiab extraction, thiab cov zaub yog yooj yim dua thiab haum rau loj-scale input ntau lawm.
Cov txheej txheem ntau lawm ntawmsulforaphane
1. Enzymatic digestion
Enzymatic digestion yog tus nqi-zoo tshaj plaws los tsim sulforaphane. Cov kev tshawb fawb tau pom tias qhov zoo tshaj pH rau kev tsim cov sulforaphane yog 5.0-8.0 thiab lub sij hawm enzymatic digestion yog 8-12 h. Cov txheej txheem rho tawm tuaj yeem ua kom zoo dua los txhim kho tus nqi rho tawm, thiab ntxiv cov kua txiv hmab txiv ntoo rau hauv cov zom zom zom tsis tuaj yeem pab txhawb kev zom zaub mov, ntxiv rau qhov sib ntxiv ntawm qee cov khoom lag luam ruaj khov xws li VC lossis Zn2, uas tuaj yeem txhim kho tau zoo. Cov txiaj ntsig ntawm sulforaphane.
2. Kev rho tawm
① Extraction tuaj yeem muab faib ua cov kuab tshuaj extraction thiab ultrasound-pab extraction, tab sis vim cov kuab tshuaj extraction muaj kev txwv ntawm tus neeg sawv cev extraction, tus nqi extraction ntawm siab toxicity ntawm cov extractant tsis muaj feem xyuam thaum lub extracted radish sulfur yog siv los ua zaub mov lossis tshuaj, Tsis tas li ntawd, nws tseem yuav ua rau muaj kuab paug rau ib puag ncig, thiab cov hmoov ua tiav kuj tseem muaj qhov saj ntawm cov kuab tshuaj, txo qhov kev xav ntawm cov neeg siv khoom zoo.
② Yog li ntawd, txoj kev tseem ceeb siv tam sim no yog ultrasound-pab extraction, piv nrog cov tshuaj hnyav extraction txoj kev, ultrasound-pab extraction muaj ib tug me me npaum li cas ntawm cov kuab tshuaj, thiab ultrasound-pab enzymatic digestion es tsis txhob sawv enzymatic digestion ntawm chav tsev kub txuag feem ntau. ntawm lub sijhawm. Raws li cov kws tshawb fawb, cov xwm txheej zoo tshaj plaws rau kev siv ultrasound-pab extraction yog 80 feem pua ethanol ua cov kuab tshuaj rau ultrasound extraction, uas tau xaiv vim nws yooj yim rau qhov chaw thiab muaj kev nyab xeeb ntxiv rau hauv ntau lawm, 30 min rau ultrasound extraction, thiab 2 zaug rau ultrasound extraction.
③ Cov kauj ruam rho tawm yog ib kauj ruam tseem ceeb hauv cov txheej txheem kom tau txais sulforaphane. Yog tsis muaj enzymatic digestion, cov ntsiab lus ntawm sulforaphane yuav luag xoom.
3. Kev kho kom zoo
Tom qab rho tawm cov sulforaphane los ntawm cov kauj ruam saum toj no, cov extract yuav tau concentrated thiab qhuav kom tau cov sulforaphane crude, ces tom qab crushing thiab sieving, thiab kuaj lub ntim, tiav lawm.Sulforaphane hmoovtuaj yeem tau.
Lwm txoj kev ntawm extracting sulforaphane
1. Tshuaj synthesis method
Cov tshuaj tiv thaiv kab mob yog qhov nyuaj rau kev tswj, cov kauj ruam yog tedious thiab tus nqi siab, yog li nws tsis tshua muaj siv hauv kev xyaum. Raws li cov ntaub ntawv tseem ceeb, cov txheej txheem tshuaj synthesis ntawm sulforaphane feem ntau yog siv los ua ke S-methyltetrahydrothiopheneium tetrafluoroborate nrog tetrahydrothiophene, methyl iodide, thiab sodium tetrafluoroborate ua raw cov ntaub ntawv, thiab tom qab ntawd hnov mob nrog sodium azide los ua 1-azido-( {3}}methylsulfonyl)butane, thiab tau txais isothiocyanate los ntawm Staudinger cov tshuaj tiv thaiv, thiab thaum kawg tau radish sulforaphane los ntawm H2O2oxidation.
2. Semi-synthetic txoj kev
Nws yog ib qho kev sib xyaw ntawm cov tshuaj synthesis thiab enzymatic txoj kev, siv glucoraphanin structural analogs raws li raw khoom uas pheej yig thiab yooj yim kom tau, tab sis ua ntej extracting thioglucoside yuav tsum mus los ntawm enzyme inactivation kev kho mob, ntxiv rau ntxiv exogenous thioglucosidase thiab tom qab ntawd los ntawm hydrolysis kom tau. sulforaphane.
Ob txoj kev no yuav tsum tau siv ntau cov kuab tshuaj organic, uas tuaj yeem ua rau muaj kuab paug rau ib puag ncig thiab tsis haum rau kev siv sulforaphane hauv kev xyaum.
Yog xav paub ntxiv txais tos hu rau peb ntawmsales@botanicalcube.com